BIOMEDICA Quality CYTOKINE KITS for affordable & efficient research

BIOMEDICA Quality CYTOKINE KITS for affordable & efficient research

My name is Lan Chi. I am part of the Biomedica assay quality management team. All our ELISA kits undergo a stringent quality control process. This ensures reliable and consistent results. We are located right in the heart of Europe.  I am happy to introduce our new human Cytokine ELISA Kits  – developed & manufactured by Biomedica 

BIOMEDICA Quality CYTOKINE KITS for affordable & efficient research.

 

IL-6 (Interleukin-6)                         high sensitivity – detectable levels

VEGF                                                       low sample volume – 10µl

ANGIOPOIETIN-2                          optimized assay range – no predilution

 All kits include color coded, ready to use prediluted standards and controls.

USER-FRIENDLY & RELIABLE ELISA kits

• reproducible & consistent results
• Use of WHO International Standards – for kit calibration & harmonization 
• FULL VALIDATION PACKAGE

 

WE VALIDATE OUR KITS ACCORDING to INTERNATIONAL QUALITY STANDARDS 

 

Biomedica ´s ELISA assay kits are validated according to international quality guidelines (ICH, EMEA, FDA).  You can find the assay validation data on the respective product pages of our website.  

DILUTION LINEARITY (PARALLELISM): CONSISTENT RECOVERY IN DILUTED SAMPLES

Our validation data include the following experiments: e.g. parallelism (dilution of samples containing elevated endogenous levels of the protein of interest) and dilution linearity (dilution of samples containing levels of endogenous and recombinant protein of interest).

WITHIN-RUN AND IN-BETWEEN RUN PRECISION: PRECISE & REPRODUCIBLE RESULTS WITHIN AND ACROSS LOTS

Our lab team also validates our assays for precision and accuracy. The precision of our assays is determined by measuring  samples of known concentrations a number of times with one kit lot (within-run precision) and with two different kit lots (in-between run precision). 

Finally, we check the stability of the analyte in the respective sample matrix.

STABILITY – Sample stability and stability of kit components

We take measures to ensure the stability of all component substances that are included in the kits. Accordingly, the stability of all assay components is tested during kit development. Furthermore, we expose real samples to several freeze-thaw cycles and also leave samples at room temperature for at least 24 hours. Hence, with these experiments we are able to determine sample stability after temperature stress. 

Related information:

Our Biomedica human Angiopoietin-2 ELISA  (cat.no. BI-ANG2) has been cited in the following publication:

Angiopoietin-2 predicts all-cause mortality in male but not female end-stage kidney disease patients on hemodialysis.

Chu C et al., Nephrol Dial Transplant. 2022 ;  23;37(7):1348-1356.

Particularly, a detailed description of Biomedica´s Angiopoietin-2 ELISA is shown in the following poster .  

Check out the validation data of our human IL-6 ELISA