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Cell Proliferation & Cytotoxicity Assay
Cell proliferation assays are widely used in cell biology. The EZ4U kit is a simple and reliable non-radioactive two to five-hour test. The assay employs non-toxic tetrazolium salts, which are reduced to colored formazan. As this reduction process requires functional mitochondria, which are inactivated within a few minutes after cell death, this method provides an excellent tool for the discrimination between living and dead cells. The assay offers advantages over conventional dye or 3H incorporation assays. Due to its soluble end products it is easier and faster to perform than other non-radioactive cell viability assays. As the assay procedure is identical to the thymidine incorporation procedure, no changes in test protocols are necessary. An important benefit is the possibility of an ongoing cultivation after cell number determination and easy to adapt incubation times due to the non-toxic substrate. The EZ4U cell proliferation & cytotoxicity assay is well suited for a variety of biological tests, where cell viability is of importance. Click here to learn more.
Cell Proliferation & Cytotoxicity Assay
√ Non-radioactive & non-toxic
√ Reliable & Sensitive
√ Convenient single-step incubation for use on living cells
√ Widely cited in more than 220 publications
Check out our EZ4U Brochure link
Latest Citations:
Plangger A, Rath B, Hochmair M, Funovics M, Hamilton G. Transl Oncol. 2021 Dec;14(12):101230. doi: 10.1016/j.tranon.2021.101230. Epub 2021 Sep 28. PMID: 34598083; PMCID: PMC8488304.
Tolerability to non-endosomal, micron-scale cell penetration probed with magnetic particles.
Ruiz-Cánovas E, Mendoza R, Villaverde A, Corchero JL.Colloids Surf B Biointerfaces. 2021 Sep 20;208:112123. doi: 10.1016/j.colsurfb.2021.112123. Epub ahead of print. PMID: 34571468.
Fronik P, Poetsch I, Kastner A, Mendrina T, Hager S, Hohenwallner K, Schueffl H, Herndler-Brandstetter D, Koellensperger G, Rampler E, Kopecka J, Riganti C, Berger W, Keppler BK, Heffeter P, Kowol CR.J Med Chem. 2021 Aug 26;64(16):12132-12151. doi: 10.1021/acs.jmedchem.1c00770. Epub 2021 Aug 17. PMID: 34403254; PMCID: PMC8404199.
Dyrkheeva NS, Filimonov AS, Luzina OA, Zakharenko AL, Ilina ES, Malakhova AA, Medvedev SP, Reynisson J, Volcho KP, Zakian SM, Salakhutdinov NF, Lavrik OI. Biomolecules. 2021 Jul 1;11(7):973. doi: 10.3390/biom11070973. PMID: 34356597; PMCID: PMC8301776.
Jiao LUO, Xiao-jun LI and Geon-ho LEE et al. Food Science and Technology. DOI: 10.1590/fst.89721
Interactions of BRCA1-mutated Breast Cancer Cell Lines with Adipose-derived Stromal Cells (ADSCs).
Plangger A, Haslik W, Rath B, Neumayer C, Hamilton G.J Mammary Gland Biol Neoplasia. 2021 Sep;26(3):235-245. doi: 10.1007/s10911-021-09493-4. Epub 2021 Jul 6. PMID: 34228231.