Quantify Cytokines with an ELISA Assay from BIOMEDICA

We at BIOMEDICA develop and manufacture ELISA assays to reliably quantify cytokines in biological fluids.

Our ELISA kits are rigorously validated according to international quality standards to ensure their consistency, specificity, precision, and robustness.

All of the validation data can be found on the respective product pages on our website www.bmgrp.com . 

Quantify Cytokines with an ELISA Assay from BIOMEDICA

We designed our assays to quantify the cytokine of interest in serum, plasma or cell culture supernatants.

Standardized harmonization of results with WHO reference reagents

To ensure a harmonized standardization, WHO reference reagents / standards are employed for cross-comparison of results (1)

Quantify Cytokines with an ELISA Assay from BIOMEDICA

IL-6 ELISA (cat. no. BI-IL6)

The IL-6 (Interleukin-6) ELISA kit from BIOMEDICA is a conventional  sandwich ELISA kit. The assay incorporates two epitope-mapped antibodies that specifically bind to human IL-6 in the respective samples.

Capture antibody : is a  recombinant IL-6 antibody that is specific for human IL-6, which is pre-coated on a 96-well microtiter plate. 

Detection antibody: is a polyclonal IL-6 antibody (labeled with streptavidin-HRPO), which is specific for human IL-6.

• • Assay method: sandwich ELISA
  • Sample types: serum, plasma, cell-culture, urine
  • Sample volume: 100µl / well
  • Assay time: 4.5 hrs
  • Standard range: 0; 3.1 – 200 pg/ml
  • Sensitivity: LLOQ: 0.78 pg/ml (measurable concentrations in both serum AND plasma samples!),  LOD: 0.28 pg/ml
  • Precision: in-between-run (n=9 ): ≤ 6 % CV, within-run (n=3): ≤ 7 % CV
  • Specificity:  the ELISA recognizes endogenous (natural) and recombinant and human IL-6
  • Standard matrix: 8 x pre-diluted standards, lyophilized,  in a serum based matrix containing recombinant human Il-6
  • Controls: 1x high and 1x low control included in the kit

 

VEGF ELISA (cat. no. BI-VEGF)

The VEGF (Vascular Endothelial Growth Factor) ELISA kit from BIOMEDICA is a conventional sandwich ELISA kit. The assay incorporates two epitope-mapped antibodies that specifically bind to human VEGF in the respective samples.

Capture antibody is a recombinant VEGF antibody that is specific for human VEGF, which is pre-coated on a 96-well microtiter plate,  recognizing a structural epitope in the conserved receptor binding- site of the VEGF peptide. All VEGF isoforms are detected.

Detection antibody: is a polyclonal VEGF antibody (labeled with streptavidin-HRPO), which is specific for human VEGF. The antibody recognizes multiple linear epitopes that are concentrated in the first 120 amino acids in the N-terminal region of the VEGF molecule.   

• Assay method: sandwich ELISA
• Sample types: serum, plasma, cell-culture, urine
• Sample volume: 10µl / well
• Assay time: 4.5 hrs
• Standard range: 0; 31.2 – 2000 pg/ml
• Sensitivity: LLOQ: 15.6 pg/ml); LOD: 2.5 pg/ml
• Precision: in-between-run: (n=3): ≤ 6 % CV, Within-run (n=3): ≤ 3 % CV
• Specificity: the ELISA recognizes endogenous (natural) human VEGF including all circulating VEGF isoforms including VEGF165b and recombinant VEGF
• Standard matrix: 8 x vials of pre-diluted standards, lyophilized, in a serum based matrix containing recombinant human VEGF
• Controls: 2 controls (high and low) included in the kit

 

ANGIOPOIETIN-2 ELISA (cat. no. BI-ANG2)

The ANG2 (Angiopoietin-2) ELISA kit from BIOMEDICA  is a conventional sandwich ELISA kit. The assay incorporates two epitope-mapped antibodies that specifically bind to human Angiopoietin-2 in the respective samples.

Capture antibody is a recombinant ANG2 antibody that is specific for human VEGF, which is pre-coated on a 96-well microtiter plate. The capture antibody has a structural epitope that binds to the receptor binding site of human Angiopoietin-2. The receptor binds to TEL / TIE2 protein.

Detection antibody: is a polyclonal ANG2 antibody (labeled with streptavidin-HRPO), which is specific for human ANG2. The antibody recognizes several linear epitopes that are distributed over the entire Angiopoietin-2 molecule.  

• Assay method: sandwich ELISA
• Sample types: serum, plasma, cell-culture, urine
• Sample volume: 20µl / well
• Assay time: 3.5 hrs
• Standard range: 0; 0.68 – 21.96 ng/ml (= 0 ; 12.5 – 400 pmol /l  (conversion 1 pmol/l = 54.9 pg/ml (MW: 54.9 kDa)
• Sensitivity: LLOQ : 0.34 ng/ml (=6.3 pmol/l); LOD: 0.20 pg/ml (= 3.7 pmol/l)
• Precision: In-between-run (n=9): ≤ 6 % CV, Within-run (n=3): ≤ 8 % CV
• Specificity: the ELISA recognizes endogenous (natural) human ANG2 and recombinant ANG2.  Most probably all three ANG2 isoforms are detected by the assay, as determined by epitope mapping and analysis of the ANG2 sequence. This ELISA assay does not detect ANG1.
• Standard matrix: 8 x vials of pre-diluted standards, lyophilized, in a serum based matrix containing recombinant human ANG2.
• Controls: 2 controls (high and low) included in the kit

 

Mouse /rat Angiopoietin-2 ELISA (cat. no. BI-ANG2MR) also available

Related publications

1. International reference reagents
2. Angiopoietin-2 Promotes Pathological Angiogenesis and Is a Therapeutic Target in Murine Nonalcoholic Fatty Liver Disease. Lefere S, Van de Velde F, Hoorens A, Raevens S, Van Campenhout S, Vandierendonck A, Neyt S, Vandeghinste B, Vanhove C, Debbaut C, Verhelst X, Van Dorpe J, Van Steenkiste C, Casteleyn C, Lapauw B, Van Vlierberghe H, Geerts A, Devisscher L. Hepatology. 2019 Mar;69(3):1087-1104. doi: 10.1002/hep.30294. Epub 2019 Feb 12. PMID: 30259536.