Serologic Testing for Lyme Disease

Lyme disease is a bacterial infection that is spread to humans by infected ticks. It is also known as Lyme Borrelioses caused by the borrelia bacteria. Symptoms often include fever, headache, fatigue, and an expanding skin rash.  If left untreated, the infection can spread to various parts of the body, affecting joint, heart, and the nervous system.

Lyme disease is the most common vector-borne disease in temperate zones of the northern hemisphere (1-3).

Early diagnosis and treatment are essential for effectively managing Lyme Borreliosis.

Serologic Testing for Lyme Disease

BIOMEDICA offers sensitive and specific ELISA Assay Kits for the quantitative determination of Borrelia IgG and IgM antibodies in human serum, plasma and cerebrospinal fluid.

Our BORRELIA  Enzyme immunoassays utilize recombinant antigens for the detection of IgG and IgM antibodies against the immunodominent antigens of the three genospecies: Borrelia burgdorferi sensu stricto, Borrelia afzelii and Borrelia garinii.

Borrelia IgG ELISA | BI-21032 

The use of recombinant Antigens offers the following advantages

• High sensitivity and specificity confirmed by clinical samples
• Standardized protocols for serum, plasma, CSF
• For manual and automated testing
• CE -marked for IVD use in the EU
• Widely cited

 

OF NOTE:

To improve the diagnostic specifity, the Biomedica Borrelia IgG ELISA microwell strips are coated with the following recombinant antigens:

• p21 = OspC (outer surface protein C): B. burgdorferi sensu stricto (B31), B. garinii (20047)
• p18: B. afzelii (pKo)
• p100: B. afzelii (pKo)
• VIsE: fusion protein of different Borrelia genospecies

 

Borrelia IgM ELISA | BI-21042

The use of recombinant Antigens offers the following advantages

• No extra RF stripping necessary
• High sensitivity and specificity confirmed by clinical samples
• Standardized protocols for serum, plasma, CSF
• For manual and automated testing
• CE -marked for IVD use in the EU
• Widely cited

 

OF NOTE:

To improve the diagnostic specifity, the Biomedica Borrelia IgM ELISA microwell strips are coated with the following recombinant antigens:

• p21 = OspC (outer surface protein C): B. afzellii (pKo)
• p21 = OspC (outer surface protein C): B. garinii (20047)
• p41/I = (inner part of flagellin): B. bavariensis (pBi)
• VIsE: fusion proteins of different Borrelia genospecies 

 

Literature

1. Lyme Disease Pathogenesis. Coburn J, Garcia B, Hu LT, Jewett MW, Kraiczy P, Norris SJ, Skare J. Curr Issues Mol Biol. 2021;42:473-518. doi: 10.21775/cimb.042.473. PMID: 33353871.
2. A Comprehensive Review of Lyme Disease: A Focus on Cardiovascular Manifestations. Wu M, Mirkin S, McPhail MN, Wajeeh H, Nagy S, Florent-Carre M, Blavo C, Demory Beckler M, Amini K, Kesselman MM. Cureus. 2024 May 21;16(5):e60821. doi: 10.7759/cureus.60821. PMID: 38910626; PMCID: PMC11190629.
3. The Epidemiology of Lyme Borreliosis in Europe: An Updated Review on a Growing Public Health Issue. Stark JH, Pilz A, Jodar L, Moïsi JC.Vector Borne Zoonotic Dis. 2023;23(4):139-141. doi: 10.1089/vbz.2022.0068. PMID: 37071398.